Includes bibliographical references and index.

The advent of DNA sequencing -- Chain termination sequencing: preparing the single stranded DNA template -- Chain termination sequencing: carrying out the strand synthesis reactions -- Chain termination sequencing: running the gel and reading the sequence -- Chemical degradation sequencing.

DNA sequencing is one of the most important techniques in molecular biology today. Everyone, at some time, has to sequence some DNA - whether just a few hundred base pairs, or a few megabases as part of a major sequencing project. Although it is widely practised, until now there did not exist
a practical guide for the absolute beginner. Written in an accessible and informal style, this book answers the important questions: how does DNA sequencing work? What do you need to do before getting started? What does the jargon mean? What are all the reagents for? Why hasn't it worked? If you
have ever asked any of these questions, DNA Sequencing: The Basics is for you.