Determination of cytoprotective activity of saba (Musa x paradisiaca L.) bracts extract in ethanol-induced ulcer in sprague dawley rats / Angelica P. Basco, Grazielle Anne Manzo, Kris Angel C. Oao, and Liana Mae A. Yanguas

Research Method : Descriptive Method.

Includes bibliographical references.

Abstract -- List of tables -- List of figures --List of plates -- List of appendices -- Chapter 1. The Problem Rationale -- Chapter 2. Review of related literature -- Chapter 3. Methodology -- Chapter 4. Presentation, Analysis, and Interpretation of Data -- Chapter 5. Summary, Conclusion, and Recommendation -- Bibliography -- Appendices -- Curriculum Vitae.

Cytoprotective agents has the capability to protect, reduce or prevent gastric mucosal necrosis produced by a variety of ulcerogenic and necrotizing agents. (Szabo,S., 2014). Musa x paradisiaca L. is a monoherbaceous plants belonging to the family Musaceae, distributed throughout the tropical and subtropical countries (Abi et al., 2016). Saba (Musa x paradisiaca L.) bracts are not frequently used for pharmaceutical purposes hence this study aimed to determine the cytoprotective activity of the M. x paradisiaca L. bracts extract. The dried powder of M. x paradisiaca L. bracts were macerated using 70% methanol as the solvent for 3 days (72 hours) and concentrated using EYELA N-1110 rotary evaporator. Thin layer chromatography was used to separate secondary metabolites present in the crude methanolic extract of the plant sample. OECD 425 guidelines were adopted for toxicity testing. The effect of Saba bracts was studied on experimentally induced ulceration in Sprague Dawley Rats. Twenty-five (25) Sprague Dawley Rats were randomly divided into 5 groups of 5 rats each. The first group served as the negative control and pre-treated with 0.9 % NSS at the dose of 5mL/kg. The second group was treated orally with sucralfate at a dose of 500mg/kg. Third, fourth and fifth groups served as test groups and were administered orally with methanolic extract of M. * paradisiaca L. bracts at a different dose level of 250mg/kg, 500mg/kg, and 750mg/kg. The rats were euthanized, and the stomach was harvested and examined for the histopathological evaluation. Plant extract dosed with 750mg/kg and 500mg/kg were found to have no significant difference with the positive control sucralfate 500mg/kg. This may signify that the cytoprotective effect of the plant extract bract extract with a dose of 750mg/kg and 500mg/kg are as effective as the positive control Sucralfate 500mg/kg.